电话：021-66137037

邮箱：genxili@shu.edu.cn

个人简介：

教授，博士生导师，国家杰出青年基金获得者。先后担任中国生物物理学会第九、十、十一届理事会理事，中国生物化学与分子生物学会第十、十一、十二届理事会理事、蛋白质专业委员会副秘书长、副主任兼秘书长，中国仪器仪表学会化学传感器专业委员会委员；《Biosensors and Bioelectronics》的Editor，以及国内外多家学术刊物的编委。

教育与工作经历

1984～1994年在南京大学化学系高分子材料化学、分析化学专业学习，分别获学士、硕士和博士学位

1994年6月在南京大学生化系做博士后

1996年5月出站任南京大学生化系任教

1996～2000年为南京大学副教授

1998～2000年分别在德国慕尼黑大学生物系、日本东北大学药学部、美国哈佛大学医学院生化与分子药学系做访问学者

1999.1～2010.5任南京大学生化系系主任

2001年至今任南京大学教授、博士生导师

2006年至今任上海大学兼职教授

2008.11～2011.10任上海大学生命科学学院院长。

主要研究领域：生化分析及生物传感

代表性研究成果

1. 基于蛋白质电化学发展新型生物传感器的研究，该成果荣获2010年教育部自然科学二等奖（第一完成人）

2. 蛋白质定量及活性表征的电化学研究，该成果荣获2014年教育部自然科学二等奖（第一完成人）

3. 生物分子界面作用过程的机制、调控及生物分析应用研究，该成果荣获2016年国家自然科学二等奖（第二完成人）

近五年代表性论文

1. Tao Gao, Tianshu Chen, Chang Feng, Xiang He, Chaoli Mu, Jun-ichi Anzai, Genxi Li*, Design and fabrication of flexible DNA polymer cocoons to encapsulate live cells, Nat. Commun., 2019, 10: 2946

2. Zhaowei Sun, Shuai Wu, Jiehua Ma, Hai Shi, Lin Wang, Anzhi Sheng, Tingting Yin, Lizhou Sun*, Genxi Li*, Colorimetric sensor array for human semen identification designed by coupling zirconium metal-organic frameworks with DNA modified gold nanoparticles, ACS Appl. Mater. Interfaces 2019, 11: 36316-36323

3. Lei Wang, Yucai Yang, Yunfei Liu, Limin Ning, Yang Xiang, Genxi Li*, Bridging exosome and liposome through zirconium-phosphate coordination chemistry: A new method for exosome detection, Chem. Commun., 2019, 55: 2708-2711

4. Jiehua Ma, Lan Xue, Meiling Zhang, Chao Li, Yang Xiang, Ping Liu*, Genxi Li*, An electrochemical sensor for Oct4 detection in human tissue based on target-induced steric hindrance effect on a tetrahedral DNA nanostructure, Biosensors and Bioelectronics, 2019, 127: 194-199

5. Shuai Wu, Yiwei Han, Lin Wang, Jinlong Li, Zhaowei Sun, Meiling Zhang, Ping Liu*, Genxi Li*, Sensor array fabricated with nanoscale metal-organic frameworks for the histopathological examination of colon cancer, Anal. Chem., 2019, 91: 10772-10778

6. Lingling Li, Hai Shi, Anzhi Sheng, Yi Yang, Liu Shi, Chao Li*, Genxi Li*, A novel method to engineer proteases for selective enzyme inhibition, Chem. Commun., 2019, 55: 14039-14042

7. Yue Huang, Ji Zheng, Lei Wang, Xuguo Duan, Yaosong Wang, Yang Xiang, Genxi Li*, Sensitive detection of chloramphenicol based on Ag-DNAzyme-mediated signal amplification modulated by DNA/metal ion interaction, Biosensors and Bioelectronics, 2019, 127: 45-49

8. Tao Gao, Lingling Li, Tianshu Chen, Liu Shi, Yang Yang, Genxi Li*, DNA-oriented shaping of cell features for the detection of rare disseminated tumor cells, Anal. Chem., 2019, 91: 1126-1132

9. Liu Shi, Chaoli Mu, Tao Gao, Tianshu Chen, Shuang Hei, Jie Yang*, Genxi Li*, DNA nanoflower blooms in nanochannels: a new strategy for miRNA detection, Chem. Commun., 2018, 54: 11391-11394

10. Shuai Wu, Chao Li, Hai Shi, Yue Huang, Genxi Li*, Design of metal-organic frameworks-based nanoprobes for multicolor detection of DNA targets with improved sensitivity, Anal. Chem. 2018, 90: 9929-9935

11. Tao Gao, Chaoli Mu, Hai Shi, Liu Shi, Xiaoxia Mao, Genxi Li*, Embedding capture-magneto-catalytic activity into a nanocatalyst for the determination of lipid kinase, ACS Appl. Mater. Interfaces 2018, 10: 59-65

12. Juan Zhang, Defeng Li, Xiquan Yue, Meiling Zhang, Ping Liu*, Genxi Li*, Colorimetric in situ assay of membrane-bound enzyme based on lipid bilayer inhibition of ion transport, Theranostics, 2018, 8: 3275-3283

13. Chao Li, Yaqin Tao, Yi Yang, Yang Xiang, Genxi Li*, In vitro analysis of DNA-protein interactions in gene transcription using DNAzyme-based electrochemical assay, Anal. Chem., 2017, 89: 5003-5007

14. Tao Gao, Bei Wang, Liu Shi, Xiaoli Zhu, Yang Xiang, Jun-ichi Anzai, Genxi Li*, Ultrasensitive quantitation of plasma membrane proteins via isRTA, Anal. Chem., 2017, 89: 10776-10782

15. Jinlong Li, Tao Gao, Shiy Gu, Jun Zhi, Jie Yang, Genxi Li*, An electrochemical biosensor for the assay of alpha-fetoprotein-L3 with practical applications, Biosensors and Bioelectronics, 2017, 87: 352-357

16. Juan Zhang, Xiaonan Wang, Tingjun Chen, Chang Feng, Genxi Li*, Electrochemical analysis of enzyme based on the self-assembly of lipid bilayer on an electrode surface mediated by hydrazone chemistry, Anal. Chem., 2017, 89: 13245-13251

17. Tao Gao, Liudi Li, Bei Wang, Jun Zhi, Yang Xiang, Genxi Li*, Dynamic electrochemical control of cell capture−and−release based on redox-controlled host−guest interactions, Anal. Chem., 2016, 88: 9996-10001

18. Yue Huang, Hao Li, Qiongxuan Fan, Lei Wang, Yao Wang, Genxi Li*, Multifunctional nanocatalyst-based ultrasensitive detection of human tissue transglutaminase 2, Biosensors and Bioelectronics, 2016, 83: 85-90

19. Yue Huang, Hao Li, Lei Wang, Xiaoxia Mao, Genxi Li*, Highly sensitive protein detection based on smart hybrid nanocomposite-controlled switch of DNA polymerase activity, ACS Appl. Mater. Interfaces, 2016, 8: 28202-28207

20. Hao Li, Yue Huang, Yue Yu, Yao Wang, Genxi Li*, Recognition-induced covalent capturing and labeling as a general strategy for protein detection, Biosensors and Bioelectronics, 2016, 80: 560-565

21. Hao Li, Yue Huang, Yue Yu, Genxi Li*, Yannis Karamanos, Self-catalyzed assembly of peptide scaffolded nanozyme as a dynamic biosensing system, ACS Appl. Mater. Interfaces, 2016, 8: 2833-2839

22. Hao Li, Weiwei Li, Fengzhen Liu, Zhaoxia Wang, Genxi Li*, Yannis Karamanos, Detection of tumor invasive biomarker using a peptamer of signal conversion and signal amplification, Anal. Chem., 2016, 88: 3662-3668

23. Chao Li, Yucai Yang, Dan Wu, Tianqi Li, Yongmei Yin, Genxi Li*, Improvement of enzyme-linked immunosorbent assay for the multicolor detection of biomarkers, Chem. Sci., 2016, 7: 3011-3016

24. Chao Li, Jiehua Ma, Qiongxuan Fan, Yaqin Tao, Genxi Li*, Dynamic light scattering (DLS)-based immunoassay for ultra-sensitive detection of tumor marker protein, Chem. Comm., 2016, 52: 7850-7853

25. Luming Wei, Xiaoying Wang, Dan Wu, Chao Li, Yongmei Yin, Genxi Li*, Proximity ligation-induced assembly of DNAzymes for simple and cost-effective colorimetric detection of proteins with high sensitivity, Chem. Comm., 2016, 52: 5633-5636

26. Jiehua Ma, Chao Li, Yaqin Tao, Chang Feng, Genxi Li*, Electrochemical detection of Nanog in cell extracts via target-induced resolution of an electrode-bound DNA pseudoknot, Biosensors and Bioelectronics, 2016, 86: 933-938

27. Yue Yu, Hao Li, Luming Wei, Liudi Li, Yitao Ding*, Genxi Li*, Electrochemical detection and distribution analysis of β-catenin for the evaluation of invasion and metastasis in hepatocellular carcinoma, Anal. Chem., 2016, 88: 3879-3884

28. Yuanyuan Zhang, Hao Li, Yue Huang, Lizhou Sun*, Genxi Li*, Peptide-Induced bio-mineralization as a bio-mimetic means of detecting proteins in a mineralizing bio-context, Nano Res., 2016, 9: 1489-1496

29. Yue Yu, Hao Li, Yucai Yang, Yitao Ding*, Zhaoxia Wang*, Genxi Li*, Evaluating tumor-associated activity of extracellular sulfatase by analyzing naturally occurring substrate in tumor microenvironment of hepatocellular carcinoma, Anal. Chem., 2016, 88: 12287-12293

30. Chao Li, Xiaoxi Li, Luming Wei, Muyun Liu, Yangyang Chen, Genxi Li*, Simple electrochemical sensing of attomolar protein using fabricated complexes with enhanced surface binding avidity, Chem. Sci., 2015, 6: 4311-4317

31. Luming Wei, Xiaoying Wang, Chao Li, Xiaoxi Li, Yongmei Yin, Genxi Li*, Colorimetric assay for protein detection based on “nano-pumpkin” induced aggregation of peptide-decorated gold nanoparticles, Biosensors and Bioelectronics, 2015, 71: 348-352

32. Yue Huang, Hao Li, Yuanyuan Zhang, Weiwei Li, Lizhou Sun, Genxi Li*, Ultrasensitive and feasibly achieved protein detection based on the integration of three signal amplification reactions via sharing a DNA sequence, Chem. Comm., 2015, 51: 11004-11007

33. Tao Gao, Fengzhen Liu, Dawei Yang, Yue Yu, Zhaoxia Wang, Genxi Li*, Assembly of selective biomimetic surface on an electrode surface: a design of nano-bio interface for biosensing, Anal. Chem., 2015, 87: 5683-5689

34. Hao Li, Yue Huang, Yue Yu, Weiwei Li, Yongmei Yin, Genxi Li*, Peptide-based method for detection of metastatic transformation in primary tumors of breast cancer, Anal. Chem., 2015, 87: 9251-9256

35. Chao Li, Yucai Yang, Luming Wei, Xiaoying Wang, Zhaoxia Wang*, Yongmei Yin*, Genxi Li*, An array-based approach to determine different subtype and differentiation of non-small cell lung cancer, Theranostics, 2015; 5: 62-70

36. Juan Zhang, Ying Liu, Jun Lv, Genxi Li*, A colorimetric method for α-glucosidase activity assay and its inhibitor screening based on aggregation of gold nanoparticles induced by specific recognition between phenylenediboronic acid and 4-aminophenyl-α-D-glucopyranoside, Nano Res., 2015, 8: 920-930

37. Yue Yu, Hao Li, Bin Zhang, Xiaoshu Pan, Xiaoli Zhu, Yitao Ding*, Genxi Li*, Peptide network for detection of tissue-remodeling enzyme in the prognosis of hepatocellular carcinoma, ACS Appl. Mater. Interfaces, 2015, 7: 4401-4405

38. Juan Zhang, Jun Lv, Xiaonan Wang, Defeng Li, Zhaoxia Wang*, Genxi Li*, Integration of chemoselective ligation with enzyme specific catalysis: Saccharic colorimetric analysis using aminooxy/hydrazine-functionalized gold nanoparticles, Nano Res., 2015, 8(12): 3853-3863

39. Juan Zhang, Ying Liu, Xiaonan Wang, Yangyang Chen, Genxi Li*, Electrochemical assay of α-glucosidase activity and the inhibitor screening in cell medium, Biosensors and Bioelectronics, 2015, 74: 666-672

近五年获发明专利授权

李根喜，张娟，崔俊慧，肖琳，测定α-淀粉酶活性的电化学传感器、其制备方法及应用，ZL201310586945.1

李根喜，李超，吴丹，卫璐明，黎天琪，一种可视化多色检测抗原-抗体反应试剂盒及使用方法，ZL201510430364.8

李根喜，李超，马洁桦，胡晓璐，一种基于动态三明治结构的电化学传感器及其制备和应用，ZL 201610225995.0

李根喜，马洁桦，李超，陶雅沁，特异性DNA假结结构修饰的金电极及制备方法和应用，ZL201610243290.1

李根喜，高涛，李金龙，杨大威，谷时雨，一种基于DNA分子级联信号放大的膜蛋白分析方法，ZL201610324390.7

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